GAD67 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Glutamic acid decarboxylase(GAD) catalyses the conversion of L-glutamic acid to the inhibitory neurotransmitter gamma-aminobutyric acid(GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. Human GAD65 cDNA encodes a Mr 65,000 polypeptide, with 585 amino acid residues, whereas human GAD67 encodes a Mr 67,000 polypeptide, with 594 amino acid residues. GAD67 gene consists of 16 exons, spread over more than 45 kb of genomic DNA. The GAD67 gene contains an additional exon(exon 0) that together with part of exon 1, specifies the 5' untranslated region of GAD67 mRNA. Human GAD67 shows 65% identity to GAD65 and is located in 2q31. GAD67 may play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.

Description:
Rabbit IgG polyclonal antibody for Glutamate decarboxylase 1 (GAD1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
67 kDa glutamic acid decarboxylase antibody, DCE1 antibody, DCE1_HUMAN antibody, EC 4.1.1.15 antibody, FLJ45882 antibody, GAD 1 antibody, GAD 67 antibody, GAD antibody, GAD-67 antibody, GAD1 antibody, GADA antibody, Glutamate decarboxylase 1(brain 67kD) antibody, Glutamate decarboxylase 1 antibody, Glutamate decarboxylase 1 brain 67kD antibody, Glutamate decarboxylase 1 brain 67kDa antibody, Glutamate decarboxylase 67 kDa isoform antibody, OTTHUMP00000041055 antibody, SCP antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human GAD67(529-543aa PQRREKLHKVAPKIK), different from the related mouse and rat sequences by two amino acids.A synthetic peptide corresponding to a sequence at the C-terminus of human GAD67(529-543aa PQRREKLHKVAPKIK), different from the related mouse and rat sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.