GAD67 polyclonal, anti-human, mouse, rat

Background:
There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (g-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.

Alternative Name:
GAD-67, 67 kDa glutamic acid decarboxylase, CPSQ1, DCE1, DCE1, EC 4.1.1.15, FLJ45882, GAD 67, GAD, GAD-67, GAD1, Glutamate decarboxylase 1 (brain, 67kDa), Glutamate decarboxylase 1, Glutamate decarboxylase 1 brain 67kD, Glutamate decarboxylase 1 brain 67kDa, Glutamate decarboxylase 67 kDa isoform, Glutamate decarboxylase, brain, 67-KD, OTTHUMP00000041055, SCP,

Application Dilution:
WB:1:500-1:1,000
IP:1:50-1:100

Specificity: GAD67 polyclonal antibody detects endogenous levels of GAD67 protein.

Immunogen:
recombinant protein

MW: 67 kDa

Swiss-Prot: Q99259(Human) P48318(Mouse) P18088(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.