GAD67 polyclonal, anti-human, rat

Quantity: 100 µg

Background:
Glutamate decarboxylase 1 (brain, 67kDa) (GAD67), also known as GAD1, is a human gene. It is mapped to 2q31.1. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Description:
Rabbit IgG polyclonal antibody for Glutamate decarboxylase 1 (GAD1) detection. Tested with WB in Human, Rat.

Synonyms:
67 kDa glutamic acid decarboxylase antibody, DCE1 antibody, DCE1_HUMAN antibody, EC 4.1.1.15 antibody, FLJ45882 antibody, GAD 1 antibody, GAD 67 antibody, GAD antibody, GAD-67 antibody, GAD1 antibody, Glutamate decarboxylase 1 (brain 67kD) antibody, Glutamate decarboxylase 1 antibody, Glutamate decarboxylase 1 brain 67kD antibody, Glutamate decarboxylase 1 brain 67kDa antibody, Glutamate decarboxylase 67 kDa isoform antibody, OTTHUMP00000041055 antibody, SCP antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.