GAPDH (Internal) polyclonal antibody, anti-human, mouse, rat, dog

Short Description:
This goat polyclonal primary antibody targets GAPDH.

Specificity:
This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Target Names:
GAPDH (Internal)

Conjugation: Unconjugated

Accession Number: NP_002037.2; NP_001243728.1

Immunogen Region: internal region

Immunogen Sequence: GVNHEKYDNSLK

Application Dilution: WB-0.03-0.1µg/ml
IHC-2µg/ml
IF-Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. 5-10µg/ml
ELISA-antibody detection limit dilution 1:160

Gene ID: 14433

Gene Symbol: Gapdh

Uniprot ID: G3P_MOUSE

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing

Concentrationion: 0.5mg/mL

Storage Instruction: Store at-20°C and avoid freeze-thaw cycl

Note: FOR RESEARCH USE ONLY (RUO).