GAPDH Polyclonal Antibody
€0.00
In stock
SKU
E-AB-40337
Catalog Number: E-AB-40337
Isotype: Rabbit IgG
Reactivity: human, mouse, rat
Applications: WB,IHC
Datasheet, Questions? Contact us!
Isotype: Rabbit IgG
Reactivity: human, mouse, rat
Applications: WB,IHC
Datasheet, Questions? Contact us!
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Research Area: Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Synonyms:
aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component
Immunogen: Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Swissprot: Q5XJ10
Gene ID: 317743
Calculated MW: 35 kDa
Observed MW: 35 kDa
Purification Method: Antigen Affinity Purification
Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3
Concentration: 0.66 mg/mL
Dilution: WB 1:3000-1:6000 IHC 1:300-1:500
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Citation(s):
https://onlinelibrary.wiley.com/doi/10.1002/cam4.1583
https://www.sciencedirect.com/science/article/pii/S1567576918309160
https://www.tandfonline.com/doi/abs/10.1080/02713683.2019.1584321
https://www.sciencedirect.com/science/article/pii/S0304394019301168
https://www.sciencedirect.com/science/article/pii/S156757691830941X
https://www.ncbi.nlm.nih.gov/pubmed/31496706
https://pubmed.ncbi.nlm.nih.gov/31394382/
https://pubmed.ncbi.nlm.nih.gov/31884082/
https://onlinelibrary.wiley.com/doi/abs/10.1002/fsn3.1707
https://www.sciencedirect.com/science/article/pii/S0006291X20312730
https://www.ncbi.nlm.nih.gov/pubmed/32003754
https://www.ncbi.nlm.nih.gov/pubmed/32566496
https://pubmed.ncbi.nlm.nih.gov/32272397/
https://assets.researchsquare.com/files/752b91bd-5876-4f2c-b2b8-1f78337cb0ee/v2/Manuscript.pdf
https://pdfs.semanticscholar.org/ba96/13d024251d8089f8af0401d7a2322108c0b6.pdf
https://pubmed.ncbi.nlm.nih.gov/32821143/
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Research Area: Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Synonyms:
aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component
Immunogen: Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Swissprot: Q5XJ10
Gene ID: 317743
Calculated MW: 35 kDa
Observed MW: 35 kDa
Purification Method: Antigen Affinity Purification
Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3
Concentration: 0.66 mg/mL
Dilution: WB 1:3000-1:6000 IHC 1:300-1:500
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Citation(s):
https://onlinelibrary.wiley.com/doi/10.1002/cam4.1583
https://www.sciencedirect.com/science/article/pii/S1567576918309160
https://www.tandfonline.com/doi/abs/10.1080/02713683.2019.1584321
https://www.sciencedirect.com/science/article/pii/S0304394019301168
https://www.sciencedirect.com/science/article/pii/S156757691830941X
https://www.ncbi.nlm.nih.gov/pubmed/31496706
https://pubmed.ncbi.nlm.nih.gov/31394382/
https://pubmed.ncbi.nlm.nih.gov/31884082/
https://onlinelibrary.wiley.com/doi/abs/10.1002/fsn3.1707
https://www.sciencedirect.com/science/article/pii/S0006291X20312730
https://www.ncbi.nlm.nih.gov/pubmed/32003754
https://www.ncbi.nlm.nih.gov/pubmed/32566496
https://pubmed.ncbi.nlm.nih.gov/32272397/
https://assets.researchsquare.com/files/752b91bd-5876-4f2c-b2b8-1f78337cb0ee/v2/Manuscript.pdf
https://pdfs.semanticscholar.org/ba96/13d024251d8089f8af0401d7a2322108c0b6.pdf
https://pubmed.ncbi.nlm.nih.gov/32821143/
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