Gastrin polyclonal, anti-human, rat

Quantity: 50 µl

Background:
Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17.

Synonyms:
GAS, GAST

Isotype: Guinea Pig Polyclonal

Immunogen:
Synthetic human gastrin 1 conjugated to BSA

Form: Serum containing 0.1% sodium azide

Specificity:
The antibody can be used for the diagnosis of gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum. Absorption with 10-100 ?g gastrin 1-34. gastrin 1-17, CCK 8 and CCK 4 per ml antiserum abolishes the staining, while CCK 10-20 does not.

Reactivity: human, rat

Applications: Immunofluorescence, Immunohistochemistry, suitable for frozen and paraffin-embedded tissue, no proteolytic treatment

Working DIlution: 1:40 to 1:75

Positive Control:
Formalin-fixed paraffin sections of rat antrum

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Mulder, H. et al. (1994) Gastroenterology 107 (3): 712-719
- Portela-Gomes G. et al. (1997) J. Histochem. & Cytochem. 45: 815-822