GATA4 polyclonal, anti-human, mouse, rat

Background:
Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

Alternative Name:
GATA4, ASD2, GATA 4, GATA binding protein 4, GATA-binding factor 4, gata4, GATA4, MGC126629, Transcription factor GATA 4, Transcription factor GATA-4, Transcription factor GATA4, VSD1,

Application Dilution:
WB:1:500-1:1,000
ICC:1:50-1:200
FC:1:50-1:100

Specificity: GATA4 polyclonal antibody detects endogenous levels of GATA4 protein.

Immunogen:
recombinant protein

MW: 54 kDa

Swiss-Prot: P43694(Human) Q08369(Mouse) P46152(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.