GCAT Polyclonal Antibody

Background:
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.

Research Area: Cancer, Metabolism, Signal transduction

Synonyms:
2 amino 3 ketobutyrate CoA ligase , 2 amino 3 ketobutyrate coenzyme A ligase, mitochondrial, 2-amino-3-ketobutyrate coenzyme A ligase, AKB ligase, Aminoacetone synthase, GCAT, Glycine acetyltransferase, glycine C acetyltransferase , glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase), KBL, KLB, mitochondrial

Immunogen: Fusion protein of human GCAT

Swissprot: O75600
Gene Accession: BC014457

Calculated MW: 45 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 1.02 mg/mL
Dilution: WB 1:500-1:2000, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.