GCK Polyclonal Antibody

Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Research Area: Cancer, Metabolism, Signal Transduction

Synonyms:
ATP:D-hexose 6-phosphotransferase, FGQTL3, GCK, GK, GLK, Glucokinase, Hexokinase D pancreatic isozyme, Hexokinase type IV, Hexokinase-4, Hexokinase-D, HHF3, HK IV, HK4, HKIV, HXK4, HXKP, LGLK, MODY2

Immunogen: Recombinant Mouse Glucokinase protein

Swissprot: P52792

Calculated MW: 52 kDa
Observed MW: 52 kDa

Purification Method: Antigen Affinity Purification

Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.

Concentration: 1 mg/mL
Dilution: WB 1:1000-1:2000 IHC 1:100-1:200 IF 1:100-1:400

Storage:
Store at -20°C. Avoid freeze / thaw cycles.