GCLC polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Description:
Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit (GCLC) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
EC 6.3.2.2 antibody, Gamma ECS antibody, Gamma GCS antibody, Gamma glutamylcysteine synthetase antibody, Gamma-ECS antibody, Gamma-glutamylcysteine synthetase antibody, GCL antibody, GCLC antibody, GCLM antibody, GCS antibody, GCS Heavy Chain antibody, GLCL antibody, GLCLC antibody, GLCLR antibody, Glutamate cysteine ligase catalytic subunit antibody, Glutamate cysteine ligase(gamma glutamylcysteine synthetase) regulatory(30.8kD) antibody, Glutamate cysteine ligase catalytic subunit antibody, Glutamate cysteine ligase modifier subunit antibody, Glutamate cysteine ligase regulatory protein antibody, Glutamate cysteine ligase regulatory subunit antibody, Glutamate--cysteine ligase catalytic subunit antibody, GSC light chain antibody, GSH1_HUMAN antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC(7-24aa GSPLSWEETKRHADHVRR), different from the related rat and mouse sequences by one amino acid.A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC(7-24aa GSPLSWEETKRHADHVRR), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.