GCLC polyclonal, anti-human, rat

Background:
The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. ,

Alternative Name:
GCLC, GLCL, GLCLC, EC 6.3.2.2, Gamma ECS, Gamma glutamylcysteine synthetase, Gamma-ECS antibod, Gamma-glutamylcysteine synthetase, GCL, Gclc, GCS, GCS heavy chain, GLCL, GLCLC, Glutamate cysteine ligase catalytic subunit, Glutamatecysteine ligase catalytic subunit, GSH1,

Application Dilution:
WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Specificity: GCLC polyclonal antibody detects endogenous levels of GCLC protein.

Immunogen:
Recombinant protein

MW: 73 kDa

Swiss-Prot: P48506(Human) P19468(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.