GCLC Polyclonal Antibody

Background:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Research Area: Cancer, Cell Biology, Metabolism, Signal Transduction

Synonyms:
EC 6.3.2.2, Gamma ECS, Gamma glutamylcysteine synthetase, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCL, Gclc, GCS, GCS heavy chain, GLCL, GLCLC, Glutamate cysteine ligase catalytic subunit, Glutamate--cysteine ligase catalytic subunit, GSH1

Immunogen: Fusion protein of human GCLC

Swissprot: P48506
Gene Accession: BC022487

Calculated MW: 73 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.8 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.