GCN2 polyclonal, anti-mouse, rat

Quantity: 100 µg

Background:
EIF2AK4(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz(2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al.(1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al.(2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation(late LTP or L-LTP), which was dependent on transcription and translation.

Description:
Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 4 (EIF2AK4) detection. Tested with WB in Mouse, Rat.

Synonyms:
E2AK4_HUMAN antibody, Eif2ak4 antibody, Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody, Eukaryotic translation initiation factor 2-alpha kinase 4 antibody, GCN2 antibody, GCN2 eIF2alpha kinase antibody, GCN2 like protein antibody, GCN2-like protein antibody, KIAA1338 antibody, MGCN2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2(868-886aa ATDHLAFTAEGKQDDQAGD), different from the related rat sequence by one amino acid.A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2(868-886aa ATDHLAFTAEGKQDDQAGD), different from the related rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse.
Predicted to work with: rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.