GEF H1 polyclonal, anti-human, mouse, rat

Background:
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA.

Alternative Name:
GEF-H1, ARHGEF2, KIAA0651, LFP40, AA408978, ARHG2, ARHG2, ARHGEF 2, ARHGEF-2, ARHGEF2, GEF, GEF H1, GEF-H1, GEFH1, Guanine nucleotide exchange factor H1, KIAA0651, Lbcl1, Lfc, LFP40, MGC95068, Microtubule-regulated Rho-GEF, mKIAA0651, P40, Proliferating cell nucleolar antigen p40, Protein GEF-H1, Rho guanine nucleotide exchange factor 2, rho/rac guanine nucleotide exchange factor (GEF) 2, rho/rac guanine nucleotide exchange factor 2, rho/rac guanine nucleotide exchange factor,

Application Dilution:
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: GEF H1 polyclonal antibody detects endogenous levels of GEF H1 protein.

Immunogen:
Recombinant protein corresponding to C-terminal human GEF H1.

MW: 111 kDa

Swiss-Prot: Q92974(Human) Q60875(Mouse) Q5FVC2(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.