General 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Hydroxydeoxyguanosine(8-OHdG) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 8-Hydroxydeoxyguanosine(8-OHdG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-Hydroxydeoxyguanosine(8-OHdG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway, Tumor immunity, Infection immunity, Endocrinology, Hematology, Hepatology,

Alternative Names:
8OHdG, 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine, 7,8-Dihydro-8-Oxodeoxyguanosine, 8-Hydroxy-2'-Deoxyguanosine, 8-Oxo-DG, 7,8-dihydrodeoxyguanosine

Sample Type:
serum, plasma and other biological fluids

Assay Type: Competitive Inhibition

Standard: 6000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only