General DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,25-Dihydroxyvitamin D3(DHVD3) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 1,25-Dihydroxyvitamin D3(DHVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,25-Dihydroxyvitamin D3(DHVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway, Bone metabolism

Alternative Names:
DH-VD3, Calcitriol, Rocaltrol, Calcijex, 1,25-(OH)2D3, 1,25-Dihydroxycholecalciferol, 1,25-Dihydroxy Vitamin D3

Sample Type:
serum, plasma and other biological fluids

Assay Type: Competitive Inhibition

Standard: 10000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only