General rT3 (Reverse Triiodothyronine) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Reverse Triiodothyronine(rT3) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reverse Triiodothyronine(rT3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reverse Triiodothyronine(rT3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity, Endocrinology, Hormone metabolism, Autoimmunity

Alternative Names:
RT3, r-T3, Reverse T3, (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid, 3,3',5'-Triiodo-L-thyronine

Sample Type:
serum, plasma and other biological fluids

Assay Type: Competitive Inhibition

Standard: 6000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only