GFAP (Astrocyte & Neural Stem Cell Marker) (clone ASTRO/789), anti-human, mouse, rat, cow, pig, rabbit, chicken

GFAP (Astrocyte & Neural Stem Cell Marker) (clone ASTRO/789), anti-human, mouse, rat, cow, pig, rabbit, chicken

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In stock
SKU
ARP-24-2670-MSM2
Catalog Number: 24-2670-MSM2
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1
Decription:
This monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Synonyms: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Clone: ASTRO/789

Isotype: Mouse IgG1

Immunogen: Recombinant GFAP protein

Reactivity: human, mouse, rat, cow, pig, rabbit, chicken. Others not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: Brain or Astrocytoma

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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