GFR alpha-1 human recombinant protein

Background:
GFR alpha-1, also known as GDNF family receptor alpha-1, belongs to the GDNFR family. It is a glycosylphosphatidylinositol (GPI)-linked receptor found on the cell surface, acting as a receptor for both GDNF and NTN and function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. It bind GDNF strongly and activate RET tyrosine kinase and is also essential for the development of kidneys and the enteric nervous system. Recombinant human GFR alpha-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names:
GDNF family receptor alpha-1 isoform b, GDNF receptor alpha-1, GDNF R alpha-1, GFR alpha1, GFR-ALPHA-1, GFRa-1, GFRalpha-1, GFRA1, GDNFR, GDNFRA, RET1L, RETL1, TRNR1, RHDA4, RET ligand, TGF-beta-related neurotrophic factor receptor 1

Domain: 25-424aa
Sequence:
DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLAS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRV VPFISVEHIP KGNNCLDAAK ACNLDDICKK YRSAYITPCT TSVSNDVCNR RKCHKALRQF FDKVPAKHSY GMLFCSCRDI ACTERRRQTI VPVCSYEERE KPNCLNLQDS CKTNYICRSR LADFFTNCQP ESRSVSSCLK ENYADCLLAY SGLIGTVMTP NYIDSSSLSV APWCDCSNSG NDLEECLKFL NFFKDNTCLK NAIQAFGNGS DVTVWQPAFP VQTTTATTTT ALRVKNKPLG PAGSENEIPT HVLPPCANLQ AQKLKSNVSG NTHLCISNGN YEKEGLGASS

UniProt No.: P56159
NCBI Accession No.: NP_665736.1

Molecular Weight: 70.8 kDa (633aa)
Concentration: 0.5 mg/ml (determined by absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 90% by SDS-PAGE
Bioactivity: Measured by its binding ability in a functional ELISA with Human GDNF (CAT# ATGP3942). The ED50 range ≤ 50 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: hIgG-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.