Girdin (phospho Ser-1416) polyclonal, anti-human, mouse, rat

Background:
Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

Background References
Simpson, F. (2005) Traffic. 6(6):442
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Lin, C. (2011) Sci Signal. 4(192): ra64
Lin, C. et al. (2014) Mol Biol Cell. 25(22):3654
Bhandari, D. et al. (2015)Proc Natl Acad Sci. 112(35):E4874
Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55

Immunogen: Phospho-Girdin (Ser-1416) synthetic peptide (coupled to carrier) corresponding to amino acid residues around serine 1416 in human Girdin. This sequence is identical in rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.

Specificity: The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1416) peptide before affinity purification using phospho-Girdin (Ser-1416) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with EGF or calyculin A. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate Girdin.

Application dilution:
ELISA: 1:1000
WB: 1:500

End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.