Girdin (phospho Ser-1674) polyclonal, anti-human, mouse, rat
€410.00
In stock
SKU
ECM-GP5781
Catalog Number: ECM-GP5781
Size: 100 μl
Isotype: rabbit polyclonal
Applications: WB, E
Reactivity: Hu, Ms, Rt
Datasheet
Questions? Contact us!
Size: 100 μl
Isotype: rabbit polyclonal
Applications: WB, E
Reactivity: Hu, Ms, Rt
Datasheet
Questions? Contact us!
Background:
Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.
Background References
Simpson, F. (2005) Traffic. 6(6):442
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Bhandari, D. et al. (2009) Mol Biol Cell. 20:1324.
Lin, C. (2011) Sci Signal. 4(192): ra64
Lin, C. et al. (2014) Mol Biol Cell. 25(22):3654
Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55
Immunogen: Phospho-Girdin (Ser-1674) synthetic peptide (coupled to carrier) corresponding to amino acid residues around serine 1674 in human Girdin. This sequence has high homology to rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.
Specificity: The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1674) peptide before affinity purification using phospho-Girdin (Ser-1674) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylate Girdin.
Application dilution:
ELISA: 1:1000
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.
Background References
Simpson, F. (2005) Traffic. 6(6):442
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Bhandari, D. et al. (2009) Mol Biol Cell. 20:1324.
Lin, C. (2011) Sci Signal. 4(192): ra64
Lin, C. et al. (2014) Mol Biol Cell. 25(22):3654
Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55
Immunogen: Phospho-Girdin (Ser-1674) synthetic peptide (coupled to carrier) corresponding to amino acid residues around serine 1674 in human Girdin. This sequence has high homology to rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.
Specificity: The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1674) peptide before affinity purification using phospho-Girdin (Ser-1674) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylate Girdin.
Application dilution:
ELISA: 1:1000
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
| Is Featured? | No |
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