Gli2 polyclonal, anti-human

Quantity: 100 µg

Background:
GLI2(Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel(Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al.(1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al.(2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

Description:
Rabbit IgG polyclonal antibody for Zinc finger protein GLI2 (GLI2) detection. Tested with WB in Human.

Synonyms:
Gli 2 antibody, GLI family zinc finger 2 antibody, GLI kruppel family member 2 antibody, GLI Kruppel family member GLI2 antibody, GLI2 antibody, GLI2_HUMAN antibody, Oncogene GLI2 antibody, Tax helper protein antibody, Tax helper protein 2 antibody, Tax responsive element 2 holding protein antibody, Tax responsive element 25 bp sequence binding protein antibody, THP 2 antibody, THP antibody, THP2 antibody, Zinc finger protein GLI2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2(1444-1463aa MLYYYGQIHMYEQDGGLENL).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.