Gli3 polyclonal, anti-human

Background:
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-3 (GLI family zinc finger 3), also known as GLI3FL (GLI3 full length protein), PHS, ACLS, GCPS, PAPA, PAPB, PAPA1 or PPDIV, is a 1,580 amino acid nuclear and cytoplasmic protein that acts as both a transcriptional activator and a repressor of the Sonic hedgehog (Shh) pathway. A member of the GLI C2H2-type zinc-finger protein family, GLI-3 is encoded by a gene that maps to human chromosome 7p14.1. Defects in the GLI-3 gene are the cause of a disorder known as Greig cephalo-poly-syndactyly syndrome (GCPS), which affects limb and craniofacial development.

Alternative Name:
GLI3-190, GLI3FL, GLI3-83, GLI3, ACLS, DNA binding protein, GCPS, Gli 3, GLI family zinc finger 3, GLI Kruppel family member GLI 3, GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome), GLI Kruppel family member GLI3, GLI3, GLI3 C-terminally truncated form, GLI3 form of 190 kDa, GLI3 form of 83 kDa, GLI3 full length protein, GLI3-190, GLI3-83, GLI3, GLI3FL, Glioma associated oncogene family zinc finger 3, Oncogene GLI3, PAP A, PAPA 1, PAPA, PAPA1, PAPB, PHS, PPD IV, PPDIV, Transcriptional activator GLI3, Transcriptional repressor GLI3R, Zinc finger protein GLI 3, Zinc finger protein GLI3,

Application Dilution:
WB:1:500-1:2,000
ICC:1:50-1:100

Specificity: Gli3 polyclonal antibody detects endogenous levels of Gli3 protein.

Immunogen:
Recombinant protein within human Gli3 aa 1300-1600.

MW: 190 kDa

Swiss-Prot: P10071(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.