GLS Polyclonal Antibody

Background:
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase. It has 3 isoforms named as KGA, GAM, GAC.

Research Area: Cancer, Metabolism, Neuroscience, Signal Transduction

Synonyms:
AAD20, DKFZp686O15119, FLJ10358, GAC, GAM, GLS, GLS1, GLSK, Glutaminase C, Glutaminase kidney isoform, Glutaminase phosphate activated, K-glutaminase, KGA, KIAA0838, L-glutamine amidohydrolase, mitochondrial

Immunogen: Synthetic peptide of human GLS

Swissprot: O94925
Gene Accession: NP001243239

Calculated MW: 73 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 1.02 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:50-1:200, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.