Glucagon polyclonal, anti-human, rat, porcine

Quantity: 50 µl

Background:
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.

Synonyms:
GCG, GLP1, GLP2, GRPP

Isotype: Guinea Pig Polyclonal

Immunogen:
Highly purified porcine pancreatic glucagon (Novo)

Form: Serum containing 0.1% sodium azide

Specificity:
The glucagons antibody can be used for the detection of pancreatic endocrine tumors. The antigen localization is cytoplasmic. Absorption with 10-100 ?l glucagon per ml diluted antiserum abolishes staining.

Reactivity: human, rat, porcine

Applications: Immunofluorescence, Immunohistochemistry, suitable for frozen and paraffin-embedded tissue, no proteolytic treatment

Working DIlution: 1:100-1:200 (PAP); 1:20 - 1:40 (FITC)

Positive Control:
Bouin-fixed paraffin sections of pancreas

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Myrsen-Axcrona U et al. (1997) Regulary Peptides 68: 165-175