Glycogen synthase 1(Phospho-S645) polyclonal, anti-human, rat

Background:
Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver. Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals -- muscle isoform (GYS1) and liver isoform (GYS2) respectively. Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649 and Ser653) inhibiting its activity. Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth.

Alternative Name:
Glycogen [starch] synthase, muscle, GYS1, GYS

Application Dilution:
WB: 1:1000~1:2000

Specificity: Glycogen synthase 1(Phospho-S645) polyclonal antibody detects endogenous levels of Glycogen synthase 1 protein only when phosphorylated at Ser645.

Immunogen:
Synthetic phosphopeptide derived from human Glycogen synthase 1 around the phosphorylation site of Serine 645.

MW: ~ 90 kDa

Swiss-Prot: P13807

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.