Glycophorin C (clone BGRL 100) Culture supernatant, anti-human
€204.00
In stock
SKU
ARP-08-9416-1
Catalog Number: 08-9416-1
Size: 0.5 ml
Isotype: Mouse IgG1 kappa light chain
Size: 0.5 ml
Isotype: Mouse IgG1 kappa light chain
Decription:
Mouse monoclonal antibody to human Glycophorin C (Cytoplasmic domain) Culture supernatant. Glycophorin C (GPC, sialoglycoprotein beta, glycoconnectin) and glycophorin D (GPD, sialoglycoprotein s) are closely related erythrocyte transmembraneous glycoproteins, which are products of the same glycophorin C gene. The GPC (Mr 40K) amino acid sequence consists of 128 amino acids which traverses the bilayer once. GPC has three domains: (i) a glycosylated N-terminal extracellular domain (residues 1-57) containing about 12 O-glycans and 1 N-glycan; (ii) a hydrophobic intramembranous domain (58-81) which spans the lipid bi-layer; and (iii) a C-terminal cytoplasmic domain (82-128). GPD is identical to GPC but lacks the first 21 NH2-terminal amino acid residues2,. GPC is coded on chromosome 2q14-q2. The functional role of GPC and GPD is in the regulation of shape and membrane mechanical properties of the red cell through the interaction of their cytoplasmic domains with membrane protein 4.1 within the cytoskeleton network. GPC carries the Gerbich blood group antigens. GPC is deficient in erythrocytes of the Leach type of Gerbich negative. The other types of Gerbich negative, Gerbich type and Yus type, have altered forms of GPC. Rare individuals of the Leach phenotype lacking GPC and GPD have elliptocytic red cells. GPC expression is not confined to erythroid cells3 as GPC has a fairly broad distribution in both erythroid and non-erythroid tissues4. Previously 50-100,000 copies of GPC per red cell were estimated. By the use of Fab fragments of monoclonal antibodies, the number of molecules per red cell has been estimated at 143,0005.
Synonyms: GPC, sialoglycoprotein beta, glycoconnectin
Clone: BGRL 100
Isotype: Mouse IgG1 kappa light chain
Immunogen: Synthetic peptide comprising of 17 amino acid residues within the cytoplasmic C-terminal domain. Glycophorin C has a fairly broad distribution in erythroid and non-erythroid tissues.
Reactivity: human
Form: Culture Supernatant
Applications: Immunoblotting, Immunohistochemistry, Immunoprecipitation
Working Dilution: Optimal dilution should be performed by user
Storage: 2-8C for immediate use, or at -20C (aliquot)
References:
1. Cartron, J.P., et al. (1990) In Blood Cell Biochemistry, Vol. 1: Erythroid Cells (ed. J.R. Harris) 299-335. Plenum Press
2. Tanner, M.J.A, et al. (1988(, Biochem J. 250, 407-414
3. Anstee, D.J. (1990) Vox Sang. 58: 1-20 (review)
4. Anstee, D.J., et al. (1992) In Biochemistry of the erythrocyte polypeptide blood group antigens, ed. Agre P & Cartron, J.P. John Hopkins University Press.
5. Smythe J et al (1994) Blood 83 6 1668-1672
6. Reid M.E. et al. (1990), Transf. Med. 1, Suppl. 1, p 66 (Abstract).
7. King et al., (1995) Brit. J. Haem., 89: 440-448
Mouse monoclonal antibody to human Glycophorin C (Cytoplasmic domain) Culture supernatant. Glycophorin C (GPC, sialoglycoprotein beta, glycoconnectin) and glycophorin D (GPD, sialoglycoprotein s) are closely related erythrocyte transmembraneous glycoproteins, which are products of the same glycophorin C gene. The GPC (Mr 40K) amino acid sequence consists of 128 amino acids which traverses the bilayer once. GPC has three domains: (i) a glycosylated N-terminal extracellular domain (residues 1-57) containing about 12 O-glycans and 1 N-glycan; (ii) a hydrophobic intramembranous domain (58-81) which spans the lipid bi-layer; and (iii) a C-terminal cytoplasmic domain (82-128). GPD is identical to GPC but lacks the first 21 NH2-terminal amino acid residues2,. GPC is coded on chromosome 2q14-q2. The functional role of GPC and GPD is in the regulation of shape and membrane mechanical properties of the red cell through the interaction of their cytoplasmic domains with membrane protein 4.1 within the cytoskeleton network. GPC carries the Gerbich blood group antigens. GPC is deficient in erythrocytes of the Leach type of Gerbich negative. The other types of Gerbich negative, Gerbich type and Yus type, have altered forms of GPC. Rare individuals of the Leach phenotype lacking GPC and GPD have elliptocytic red cells. GPC expression is not confined to erythroid cells3 as GPC has a fairly broad distribution in both erythroid and non-erythroid tissues4. Previously 50-100,000 copies of GPC per red cell were estimated. By the use of Fab fragments of monoclonal antibodies, the number of molecules per red cell has been estimated at 143,0005.
Synonyms: GPC, sialoglycoprotein beta, glycoconnectin
Clone: BGRL 100
Isotype: Mouse IgG1 kappa light chain
Immunogen: Synthetic peptide comprising of 17 amino acid residues within the cytoplasmic C-terminal domain. Glycophorin C has a fairly broad distribution in erythroid and non-erythroid tissues.
Reactivity: human
Form: Culture Supernatant
Applications: Immunoblotting, Immunohistochemistry, Immunoprecipitation
Working Dilution: Optimal dilution should be performed by user
Storage: 2-8C for immediate use, or at -20C (aliquot)
References:
1. Cartron, J.P., et al. (1990) In Blood Cell Biochemistry, Vol. 1: Erythroid Cells (ed. J.R. Harris) 299-335. Plenum Press
2. Tanner, M.J.A, et al. (1988(, Biochem J. 250, 407-414
3. Anstee, D.J. (1990) Vox Sang. 58: 1-20 (review)
4. Anstee, D.J., et al. (1992) In Biochemistry of the erythrocyte polypeptide blood group antigens, ed. Agre P & Cartron, J.P. John Hopkins University Press.
5. Smythe J et al (1994) Blood 83 6 1668-1672
6. Reid M.E. et al. (1990), Transf. Med. 1, Suppl. 1, p 66 (Abstract).
7. King et al., (1995) Brit. J. Haem., 89: 440-448
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