GM-CSF polyclonal, anti-rat

Source:
Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Anti-Rat GM-CSF specific antibody was purified by affinity chromatography employing an immobilized Rat GM-CSF matrix.

Western Blot:
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Sandwich ELISA:
To detect Rat GM-CSF by sandwich ELISA (using 100 μl/welll antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Rat GM-CSF (62-058BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.

Neutralization:
To yield one-half maximal inhibition [ND₅₀] of the biological activity of Rat GM-CSF (0.5ng/ml), a concentration of 0.09/0.14 μg/ml of this antibody is required.

Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.