GnRH-Rec (clone F1G4), anti-human, rat

Source:
A BALB/c mouse was immunized with a BSA-conjugated peptide corresponding to amino acids 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) of human GnRH receptor extracellular domain.
Fusion partner: SP2/0.

Specifications:
F1G4 reacts with GnRH receptors in the anterior pituitary. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular, and intracellular loops characteristic of G protein couple receptors. Some cancers like ovarian and breast cancers sometimes carry GnRH receptors.

Species reactivity:
Positive: human, rat.

Applications:
F1G4 specifically stains human GnRH receptors in the anterior pituitary.
ELISA: +
Frozen sections: +
Paraffin sections: Tris/EDTA
Western blot: +

Positive control: Human pituitary gland, ovarian and breast cancers.

References:
- Karande, A.A., et al., Molec. Cell. Endocrinol.114: 51-56 (1995)