Granzyme B (NK/T-Cell Lymphoma Marker) polyclonal, anti-human

Description:
Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkinā€™s Disease.

Synonyms:
Cathepsin G-like 1, CCPI, CGL1, CSPB, CTLA-1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T lymphocyte associated serine esterase 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme B, Granzyme-2, GRB, Gzmb, Human lymphocyte protein (Hlp), Lymphocyte protease, SECT, T-cell serine protease 1-3E

Isotype: Rabbit polyclonal IgG

Immunogen:
Recombinant fragment (115 Amino acid residues; aa 73-187) of human Granzyme B protein

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Reactivity: human. Others-not known

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
A431 cells. Spleen or Hodgkin’s Lymphoma.

Molecular Weight:
29-32kDa

Concentration:
0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.