GRK2 polyclonal, anti-human, mouse, rat

Background:
Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of beta-adrenergic receptor kinase: GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Alternative Name:
Beta-ARK-1, ADRBK1, BARK, BARK1, GRK2, ADRBK1, Adrenergic beta receptor kinase 1, ARBK1, BARK, BARK1, Beta adrenergic receptor kinase 1, Beta ARK 1, Beta ARK1, Beta-adrenergic receptor kinase 1, Beta-ARK-1, FLJ16718, G protein coupled receptor kinase 2, G-protein coupled receptor kinase 2, GRK2,

Application Dilution:
WB:1:1,000-1:2,000
IHC:1:50-1:200

Specificity: GRK2 polyclonal antibody detects endogenous levels of GRK2 protein.

Immunogen:
recombinant protein

MW: 80 kDa

Swiss-Prot: P25098(Human) Q99MK8(Mouse) P26817(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.