Growth Hormone (clone g3H5 ), anti-human, mouse

Decription:
Human growth hormone(hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The 20kDa variant form of human growth hormone (20kDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kDa, 191 amino acid) by the deletion of amino acid residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kDa hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.

Synonyms: GH1, Growth Hormone, GH, GH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin, GHN, hGH-N,

Clone: g3H5. Anti-human growth hormone mAb, clone g3H5, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant growth hormone.

Isotype: Mouse IgG2b kappa

Immunogen: Recombinant human growth hormone (27-217aa) purified from E. coli

Reactivity: human, mouse

Form: Purified, in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Applications: ELISA, WB (Recombinant protein)

Working Dilution: Optimal dilution should be performed by user.

NCBI Nr.: NP_000506

Concentration: 0.1 mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

References:
Wickens, et al., (1978) J. Biol Chem. 253(7)2483-2495
Chang, et al., (1978) Nature . 275,617-624
Ullrich. et al., (1977) Science 196, 1313-1319.
Tsushima, et al., (2000) Endocr J. 47; S17-21
Horan, M. et al.,(2003) Hum. Mutat.21(4), 408-423