GSK3 alpha/beta Polyclonal Antibody

Background:
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3βGSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization.

Research Area: Cancer, Neuroscience, Signal Transduction, Stem Cells

Synonyms:
GSK3A, Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3B, Glycogen synthase kinase-3 beta, GSK-3 beta, Serine/threonine-protein kinase GSK3B

Immunogen: Synthesized peptide derived from human GSK3α/β around the non-phosphorylation site of Tyr279/216.

Swissprot: P49840,P49841

Calculated MW: 51 kDa
Observed MW: 51,46 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.