GTF2A2 Polyclonal Antibody

Background:
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).

Research Area: Epigenetics and Nuclear Signaling

Synonyms:
General transcription factor IIA, General transcription factor IIA subunit 2, General transcription factor IIA, 2 (12kD subunit), General transcription factor IIA, 2, 12kDa, gtf2a2, HsT18745, T2AG, TF2A2, TFIIA 12, TFIIA, TFIIA gamma , TFIIA p12 subunit, TFIIA-12, TFIIA-gamma, TFIIA12, TFIIAS, Transcription initiation factor IIA gamma chain, Transcription initiation factor IIA subunit 2

Immunogen: Fusion protein of human GTF2A2

Swissprot: P52657
Gene Accession: BC000287

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.54 mg/mL
Dilution: IHC 1:30-1:150, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.