GTF2F1 polyclonal, anti-human, mouse, rat

GTF2F1 polyclonal, anti-human, mouse, rat

€388.00
In stock
SKU
BS7719
Catalog Number: BS7719
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC, IF

Datasheet
Background:
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.

Alternative Name:
BTF4, C76800, General transcription factor IIF 74 kDa subunit, General transcription factor IIF subunit 1, General transcription factor IIF, polypeptide 1, 74kDa, Gtf2f1, MGC94148, OTTHUMP00000237859, RAP74, T2FA_HUMAN, TF2F1, TFIIF, TFIIF-alpha, Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74,

Application Dilution: WB: 1:500~1:2000, IHC/IF: 1:50~1:200

Specificity: GTF2F1 polyclonal antibody detects endogenous levels of GTF2F1 protein.

Immunogen:
Recombinant full length Human GTF2F1.

MW: ~ 58 kDa

Swis Prot.: P35269

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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