Guinea pig brain-derived neurotrophic factor (BDNF) detection kit

Use: Used to detect the concentration of guinea pig brain-derived neurotrophic factor (BDNF) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In the microwell ELISA plate pre-coated with anti-guinea pig brain-derived neurotrophic factor (BDNF) antibody (solid phase antibody), guinea pig brain-derived neurotrophic factor (BDNF) calibrator and the sample to be tested are added, and then the biotin-labeled antibody is added. After incubation and sufficient washing, HRP-coupled avidin is added. After incubation and sufficient washing, the unbound components are removed, and a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme is formed on the solid surface of the microwell plate. TMB colorimetric solution is added to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the microplate reader. The absorbance (OD value) is positively correlated with the concentration of guinea pig brain-derived neurotrophic factor (BDNF) in the sample to be tested. The concentration of guinea pig brain-derived neurotrophic factor (BDNF) in the sample can be calculated by fitting the calibrator curve.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.