H-type 2 (CD 173) (clone 19-OLE), anti-human

Source:
A BALB/c mouse was immunized with mucin isolated from a mucinous colonic adenocarcinoma.
Fusion partner: SP2/0.

Specifications:
19-OLE reacts with H type 2 antigen, the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.

Species reactivity:
Positive: human.

Applications:
Immunocytochemistry on frozen and paraffin sections. Immunofluorescence tests.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: KG1 cells or human colorectal carcinoma.

References:
- Blood transfusion and immunohaematology, Ph Rouger, D Anstee and Ch Salmon (Eds), Arnette, France 30 (5), p. 353-720, (1987).
- Bara, J. et al. Blood transfusion and immunohaematology, Ph Rouger, D Anstee and Ch Salmon (Eds), Arnette, France 30 (5), p. 685-692, (1987).
- Marionneau, S. et al. Gasterenterology, 122: 1967-1877 (2002).
- Garcher, K. et al. Invest Ophthalmol Vis Sci.35: 1184-1191 (1994)