H2AFX (phospho-S139) polyclonal, anti-human, mouse, rat

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Synonyms: H2A.X, H2A/X, H2AX

Cellular Location: Chromosome Nucleus

Immunogen:
A phospho specific peptide corresponding to residues surrounding S139 of human Histone H2A.x

Gene Symbol: Phospho-H2AFX-S139

Gene ID: 3014

Swiss prot: P16104

Calculated MW: 15kDa

Recommended dilution:
WB 1:500-2000, IHC 1:50-100, IF 1:50-100,

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.