HDAC3 polyclonal, anti-human, mouse, rat

Background:
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

Alternative Name:
HD3, HDAC3, HD3, HDAC 3, HDAC3, HDAC3, Histone deacetylase 3, RPD3 2, RPD3, RPD3-2, SMAP45,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:50
IHC:1:50-1:200

Specificity: HDAC3 polyclonal antibody detects endogenous levels of HDAC3 protein.

Immunogen:
recombinant protein

MW: 49 kDa

Swiss-Prot: O15379(Human) O88895(Mouse) Q6P6W3(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.