HDAC8 polyclonal, anti-human, mouse, rat

Background:
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Alternative Name:
HD8, HDAC8, CDA07, CDLS5, HD 8, HD8, HDAC 8, Hdac8, HDAC8, HDACL 1, HDACL1, Histone deacetylase 8, Histone deacetylase like 1, MRXS6, RPD 3, RPD3, WTS,

Application Dilution:
WB:1:1,000-1:2,000
FC:1:50-1:100

Specificity: HDAC8 polyclonal antibody detects endogenous levels of HDAC8 protein.

Immunogen:
recombinant protein

MW: 42 kDa

Swiss-Prot: Q9BY41(Human) Q8VH37(Mouse) B1WC68(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.