Helicobacter Pylori polyclonal, anti-Helicobacter pylori

Description:
The spiral shaped bacteriumĀHelicobacter pyloriĀis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.ĀH. pyloriĀis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.ĀThis antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Synonyms:
H. pylori

Isotype: Rabbit polyclonal IgG

Immunogen:
Total sonicate of Helicobacter pylori

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Reactivity: Helicobacter pylori

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
Helicobacter pylori infected stomach biopsy

Molecular Weight:
Multiple

Concentration:
0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.