HER2/ErbB2 (clone 5B6-A9-C8), anti-transfected

Background:
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Synonyms: CD340, HER-2, HER-2/neu, HER2, MLN19, NEU, NGL, TKR1

Cellular Location: Cell membrane Cytoplasm Nucleus Nucleus Single-pass type I membrane protein perinuclear region

Immunogen:
Purified recombinant human HER2/ErbB2 protein fragments expressed in E.coli.

Gene Symbol: HER2/ErbB2

Gene ID: 2064

Swiss prot: P04626

Calculated MW: 138kDa

Recommended dilution:
WB 1:1000

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.