HEXA polyclonal, anti-mouse

Quantity: 100 µg

Background:
HEXA(hexosaminidase A(alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit(amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

Description:
Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit alpha (HEXA) detection. Tested with WB in Human.

Synonyms:
Beta hexosaminidase alpha chain precursor antibody, Beta hexosaminidase subunit alpha antibody, Beta N acetylhexosaminidase antibody, Beta N acetylhexosaminidase subunit alpha antibody, Beta-hexosaminidase subunit alpha antibody, Beta-N-acetylhexosaminidase subunit alpha antibody, Hexa antibody, HEXA_HUMAN antibody, Hexosaminidase A(alpha polypeptide) antibody, Hexosaminidase A alpha polypeptide antibody, Hexosaminidase A antibody, Hexosaminidase subunit A antibody, MGC99608 antibody, N acetyl beta glucosaminidase antibody, N acetyl beta glucosaminidase subunit alpha antibody, N-acetyl-beta-glucosaminidase subunit alpha antibody, TSD antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human HEXA(513-529aa QAQPLNVGFCEQEFEQT), different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.A synthetic peptide corresponding to a sequence at the C-terminus of human HEXA(513-529aa QAQPLNVGFCEQEFEQT), different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.