HIF-1β polyclonal, anti-human

Background:
AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. Arnt 2 (aryl hydrocarbon receptor nuclear translocator 2), also known as Hif-2b or bHLHe1, is a 712 amino acid nuclear protein that is exclusively expressed in adult brain and kidney. Containing a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains, Arnt 2 specifically recognizes the xenobiotic response element (XRE).

Alternative Name:
ARNT protein, bHLHe2, HIF-1-beta, HIF1-beta, ARNT, BHLHE2, arnT, ARNT protein, ARNT, Aryl hydrocarbon receptor nuclear translocator, bHLHe2, Class E basic helix-loop-helix protein 2, Dioxin receptor, Dioxin receptor nuclear translocator, Drnt, HIF 1 beta, HIF 1beta, HIF-1-beta, HIF1-beta, HIF1B, HIF1beta, Hypoxia Inducible Factor 1, Hypoxia inducible factor 1 beta, Hypoxia-inducible factor 1-beta, nuclear translocator, Tango,

Application Dilution:
WB:1:1,000-1:2,000
IHC:1:50-1:200

Specificity: HIF-1β polyclonal antibody detects endogenous levels of HIF-1β protein.

Immunogen:
recombinant protein

MW: 87 kDa

Swiss-Prot: P27540(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.