HIF1 bata Monoclonal Antibody

Background:
Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).

Research Area: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism

Synonyms:
arnT, ARNT protein, ARNT, Aryl hydrocarbon receptor nuclear translocator, bHLHe2, Class E basic helix-loop-helix protein 2, Dioxin receptor, Dioxin receptor nuclear translocator, Drnt, HIF 1 beta, HIF 1beta, HIF-1-beta, HIF1-beta, HIF1B, HIF1beta, Hypoxia Inducible Factor 1, Hypoxia inducible factor 1 beta, Hypoxia-inducible factor 1-beta, nuclear translocator, Tango

Immunogen: Recombinant Protein

Swissprot: P27540
Observed MW: 87 kDa

Purification Method: Protein A purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:1000-2000, IHC 1:100-200

Storage:
Store at -20°C. Avoid freeze / thaw cycles.