HIF1AN polyclonal, anti-human, mouse, rat

Background:
Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1α. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the β-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1α. This hydroxylation event blocks the association of HIF-1α with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1α.

Alternative Name:
Factor inhibiting HIF-1, Factor inhibiting HIF1, FIH 1, FIH-1, FIH1, FLJ20615, FLJ22027, HIF1AN, HIF1N_HUMAN, Hypoxia inducible factor 1 alpha inhibitor, Hypoxia inducible factor 1 alpha subunit inhibitor, Hypoxia inducible factor asparagine hydroxylase, Hypoxia-inducible factor 1-alpha inhibitor, Hypoxia-inducible factor asparagine hydroxylase, Peptide aspartate beta dioxygenase,

Application Dilution: WB: 1:500~1:2000, IHC/IF: 1:50~1:200, IP/CHIP: 1:50~1:200

Specificity: HIF1AN polyclonal antibody detects endogenous levels of HIF1AN protein.

Immunogen:
Recombinant full length Human HIF1AN.

MW: ~40 kDa

Swis Prot.: Q9NWT6

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.