His Tag (clone 2G7D9 ), anti-Tag
€0.00
In stock
SKU
BT-MCA4856
Catalog Number: BT-MCA4856
Size(s): 50μL, 100μL
Isotype: Mouse IgG1
Reactivity: Tag
Application(s): WB, IP, IHC, ICC, FCM
Datasheet
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Size(s): 50μL, 100μL
Isotype: Mouse IgG1
Reactivity: Tag
Application(s): WB, IP, IHC, ICC, FCM
Datasheet
Request Information
Background:
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
Research Area: Others
Synonyms: 6 His tag, HHHHHH tag
Immunogen: Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
Formulation: Purified antibody in PBS with 0.05% sodium azide
Storage:
4°C|-20°C for long term storage
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
Research Area: Others
Synonyms: 6 His tag, HHHHHH tag
Immunogen: Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
Formulation: Purified antibody in PBS with 0.05% sodium azide
Storage:
4°C|-20°C for long term storage
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