His-tag Polyclonal Antibody

Background:
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Alternative Names:
6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag

Immunogen: 6X His synthetic peptide conjugated to KLH.

Isotype: Rabbit

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml

Observed band: 15

GeneID: His-Tag

Storage: -20°C/1 year

NOTE: For Research Use Only