Histamine polyclonal, anti-human, rat, mouse, and most mammals

Quantity: 100 µl

Background:
Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.

Isotype: Rabbit Polyclonal

Immunogen:
Histamine conjugated to BSA

Form: Serum containing 0.1% sodium azide

Specificity:
Absorption with 10-100 ?g histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagons and histidine do not.

Reactivity: human, rat, mouse, and most mammals

Applications: Immunohistochemistry, suitable for frozen and paraffin sections

Working DIlution: 1:50-1:100 (PAP); 1:15 - 1:30 (immunofluorescence microscopy)

Positive Control:
Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach (see ref.)

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Hakansson R et al. (1986) Histochemistry 86: 5-17
- Johansson O et al. (1992) Histochem J 24: 283-287
- Axelson J et al. (1990) Gastroenterology 99: 635-640