Histone H1.0 polyclonal, anti-human, mouse, rat

Background:
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Alternative Name:
H1F0, H1 histone family member 0, H1(0), H10, H10, h1f0, H1FV, Histone H1'', Histone H1(0), Histone H1.0, Histone H10, Histone H5, MGC5241, N-terminally processed,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Histone H1.0 polyclonal antibody detects endogenous levels of Histone H1.0 protein.

Immunogen:
recombinant protein

MW: 28 kDa

Swiss-Prot: P07305(Human) P10922(Mouse) P43278(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.